Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + MLK2 Kinase Enzyme System
Recombinant human mLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. mLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.
Promega CAMKK1 Kinase Enzyme System, 10microg
CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively.
Promega HIPK3 Kinase Enzyme System, 1mg
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.
Promega AMPK (A1/B1/G2) Kinase Enzyme System, 1mg
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as master regulator of cellular energy homeostasis.
Promega BRK Kinase Enzyme System, 1mg
Recombinant full-length human BRK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain.
Promega ADP-Glo™ Kinase Assay + FGFR2 Kinase Enzyme System
Recombinant human FGFR2 (285-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. FGFR2 is a member of the fibroblast growth factor receptor family, which play a role in mitogenesis and differentiation.
Promega MET (M1250T) Kinase Enzyme System, 10microg
Recombinant human MET (point mutant M1250T; amino acids 956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega MAPKAPK5 Kinase Enzyme System, 10microg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega FGFR2 Kinase Enzyme System, 10microg
Recombinant human FGFR2 (285-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. FGFR2 is a member of the fibroblast growth factor receptor family, which play a role in mitogenesis and differentiation.
Promega ADP-Glo™ Kinase Assay + MAPKAPK5 Kinase Enzyme System
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega Kinase Selectivity Profiling System: Other/CK-1 + ADP-Glo™ Assay
Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo™ Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.
Promega ADP-Glo™ Kinase Assay + GRK5 Kinase Enzyme System
Recombinant full-length human GRK5 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family.
Promega ADP-Glo™ Kinase Assay + CDK1/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
Promega PKC alpha Kinase Enzyme System, 1mg
Recombinant full-length human PKC alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC alpha is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega AXL Kinase Enzyme System, 1mg
Recombinant human AXL (amino acids 473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. AXL is a member of the receptor tyrosine kinase family, which has oncogenic potential and is implicated in human myeloid leukemia.