Signal Transduction Reagents and Kits
Promega FLT1 Kinase Enzyme System, 1mg
Recombinant human FLT1 (amino acids 784-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FLT1, also known as fms-like tyrosine kinase 1, is a member of the VEGFR family and is related to the oncogene ROS.
Promega MLK2 Kinase Enzyme System, 1mg
Recombinant human MLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.
Promega ULK1 Kinase Enzyme System, 10microg
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ULK1 is a serine/threonine protein kinase that plays a critical role during initial stages of autophagy, a vital response to nutrient starvation.
Promega CHK1 Kinase Enzyme System, 1mg
Recombinant full-length human CHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK1 was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle.
Promega PIM2 Kinase Enzyme System, 10microg
Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues.
Promega ADP-Glo™ Kinase Assay + IKKβ Kinase Enzyme System
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + MAPKAPK3 Kinase Enzyme System
Recombinant full-length human MAPKAPK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity.
Promega LRRK2 Kinase Enzyme System, 10microg
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain and a WD40 domain.
Promega ADP-Glo™ Kinase Assay+ MAPKAPK2 Kinase Enzyme System
Recombinant human MAPKAPK2 (46-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase that is regulated via direct phosphorylation by p38 MAP kinase.
Promega MAPKAPK5 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega PLK1 Kinase Enzyme System, 10microg
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.
Promega CLK3 Kinase Enzyme System, 10microg
Recombinant full-length human CLK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK3, also known as CDC-like kinase 3, encodes a serine/threonine-type protein kinase with a non-conserved N-terminal domain.
Promega NADP/NADPH-Glo™ Assay
The NAD/NADH-Glo™ Assay is a bioluminescent, homogeneous single-reagent-addition assay for detecting total oxidized and reduced nicotinamide adenine dinucleotides and determining their ratio in biological samples or in defined enzyme reactions.
Promega ADP-Glo™ Kinase Assay + TNIK Kinase Enzyme System
Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TNIK or TRAF2 and NCK-interacting kinase are characterized by N-terminal kinase and C-terminal GCK domains that serve a regulatory function.
Promega FAK Kinase Enzyme System, 1mg
Recombinant human FAK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. FAK is involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix.